The prepared solution was filtered through a 0.22 mm pore size membrane (Millipore) and 10 ml was injected onto a reversed- phase column (Diamonsil C18, 250 mm 4.6 mm i.d.).
The prepared solution was filtered through a 0.22 mm pore size membrane (Millipore) and 10 ml was injected onto a reversed- phase column (Diamonsil C18, 250 mm 4.6 mm i.d.).