One g of pectin sample was weighed, dissolved in glass distilled water and made up to 100 mL in a volumetric flask.
25 mL of the pectin solution was transferred to a 250 mL conical flask. One g of sodium chloride was added along with six drops of Hinton's indicator.The pectin solution was titrated with 0.1 mol equi/L NaOH until the colour of the indicator changes to magenta colour and pH 7.5. To this neutralized solution, 25 mL of 0.25 mol equi/L NaOHwas added, mixed thoroughly, and allowed to stand for 30 min at room temperature. 25 mL of 0.25 mol equi/L HCl was added mixed thoroughly and titrated with 0.1 mol equi/L NaOH