We studied the applicability of intersimple sequence repeat (ISSR) polymorphism in cotton.
We found that: (i) the resolving power of agarose gels is poor relative to that provided
by sequencing gels; (ii) fluorescent labelling of ISSR amplification primers produced
numerous scorable bands; (iii) primer mixing (double priming) generated more bands than
the sum of fragments resulting from two single primers, although an unexplained dis-
appearance of several larger fragments also reproducibly occurred; (iv) ISSR fingerprinting
patterns are highly heritable; and (v) double priming ISSR is an easy and informative
genetic marker system in cotton for revealing both inter- and intraspecific variations.