mL of distilled water. Following addition of 2 drops of HCl,
samples were then centrifuged at 17,400×g for 10 min and
VFA were analyzed by gas chromatography (Hewlett
Packard 6890 Plus, Wilmington USA) according to the
method of Otto et al. (2003).
Statistical analyses
In this experiment, statistical analyses were performed
as a randomized complete block design using GLM
procedures of SAS (1996). Each pen served as the
experimental unit. The model included the effects of block
(replication) and treatment. Orthogonal contrasts were used
to separate treatment means and consisted of 1) CON vs.
CTC and 2) CTC vs. EF. In addition, CON diet was
compared to EF diets by the polynomial regression method
(Peterson, 1985) to determine linear and quadratic effects.
Variability in the data was expressed as standard error (SE)
of the mean and the chosen level of significance was 5%.
mL of distilled water. Following addition of 2 drops of HCl,
samples were then centrifuged at 17,400×g for 10 min and
VFA were analyzed by gas chromatography (Hewlett
Packard 6890 Plus, Wilmington USA) according to the
method of Otto et al. (2003).
Statistical analyses
In this experiment, statistical analyses were performed
as a randomized complete block design using GLM
procedures of SAS (1996). Each pen served as the
experimental unit. The model included the effects of block
(replication) and treatment. Orthogonal contrasts were used
to separate treatment means and consisted of 1) CON vs.
CTC and 2) CTC vs. EF. In addition, CON diet was
compared to EF diets by the polynomial regression method
(Peterson, 1985) to determine linear and quadratic effects.
Variability in the data was expressed as standard error (SE)
of the mean and the chosen level of significance was 5%.
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