The concentration of phenolic compounds in the extract was determined by the Folin-Ciocalteu method.
The absorbance of the sample and standard were measured at 765 nm and estimations were calculated from a calibration curve obtained with gallic acid. Total phenolics were expressed as mg gallic acid equivalent (GAE)/g extract.
Total flavonol was determined with Neu’s reagent solution by the method of Dai, Andary, Mondolot-Cosson, and Boubals (1995).
The absorbance of the sample and standard were measured at 410 nm and the flavonols were expressed as mg rutin equivalent (RE)/g extract.