One hundred milliliters (100ml) of potato dextrose broth (PDB) were
dispensed into separate 250 - Erlenmeyer flasks and
inoculated with 5-mm-diameter discs from the edge of 7-day-old cultures of the test antagonists maintained on PDA. Each flask was inoculated with three discs and the set up incubated at 28 ± 2o C for 7, 14, 21, and 28days. Culture filtrates were harvested in batches by filtering through Whatman No.1 filter papers. Nine milliliters (9ml) of each test filtrate were used to amend 50ml of sterile PDB. The amended broth was then inoculated with a 5-mm-diameter mycelial plug of the pathogen . Three control flasks each containing 50ml PDB amended with 9ml sterile distilled water were inoculated as in the test experiment. Inoculated flasks were incubated at 28 ± 2o C for 7days. Mycelia were harvested separately from each flask and dried to constant weight in an oven at 100o C. Percentage growth inhibition was calculated with reference to thecontrol . All the data were statistically analyzed using ANOVA and Duncan’s Multiple Range Test (DMRT) . Possible relationships between treatments and control were established.