Blood was collected from a peripheral vein (cephalic, saphenous or metatarsal veins), allowed to clot and centrifuged at 2000 g for 5 min. The serum samples were stored at 80 C until analysis
Blood was collected from a peripheral vein (cephalic, saphenousor metatarsal veins), allowed to clot and centrifuged at 2000 g for 5 min.The serum samples were stored at 80 C until analysis