2. Materials and methods
2.1. Chemicals and reagents
The mixture of aflatoxins B1, B2, G1, and G2 was bought from Trilogy Analytical Laboratory, and its standard solution was prepared in methanol. The solution was stored at 18 C until use. HPLC-grade water , acetonitrile , and methanol were used in HPLC analysis solvents. Analytical reagents such as a sodium chloride, potassium bromide, and nitric acid in aqueous solution were obtained from Samchun Pure Chemicals . Anhydrous citric acid, succinic acid, tartaric acid, and aqueous DL-lactic acid were purchased from Sigma Aldrich .
Validation of the analytical method for AFs