We optimized DART-TOF-MS for rapid and routine qualitative screening of anabolic androgenic steroids. Applicability of DART-TOF-MS for the AAS analysis was demonstrated on the AAS tablet and injection supplements, which were analyzed directly or after extraction in methanol. The direct analysis of the supplements in tablet form allowed us to omit sample pre-treatment, which as a consequence reduced the total duration of the analysis and increased the throughput of samples. This qualifies DART-TOF-MS for rapid qualitative and potentially semi-quantitative screening of AAS. Questionable samples or samples where no or a different AAS is detected by DART-TOF-MS can and should be further analyzed by a more selective method, for example GC × GC-TOF-MS. DART-TOF-MS has great potential for application in analysis of AAS, for example in forensic science and detecting adulterated and counterfeited pharmaceuticals.