Fresh sweet sorghum crops (KN Morris) grown at Heil’s Farm at Cobden, Illinois, USA, were harvested in early October of 2011. After the heads were cut and leaves removed, the stalks were stored in shade for seven days before they were squeezed by a mill to obtain fresh juice which was then cooked to attain syrup. Bottled syrup used in this study has been stored at room temperature. Before use, the dense syrup was diluted by dis- tilled and deionized water (DDW) 25 times (weight basis) and quantified by HPLC. Syrup used in fermentation studies was auto- claved at 121 ◦ C for 30 min. To understand effect of sterilization on syrup sugar composition, diluted syrup with a dilution factor of 5 was autoclaved for five times consecutively. After each autoclave, samples were withdrawn and further diluted by DDW for HPLC analysis.