reported on the free folate content (in the form of L-5-MTHF) in some commercial beers, using HPLC analysis; however, the folate content in the wine was undetectable for quantification. In the present study, our use of a microbiological assay, employing chicken pancreas conjugase pretreatment, enabled the measurement of total folate in wine, which includes both folate monoglutamate and polyglutamate forms.