Wound creation. The rats were weighed prior to the surgical procedure. The animals
were anesthetized by intra muscular injection of ketamine hydrochloride (40 mg/kg)
plus xylazine hydrochloride (5 mg/kg). Carprofen was injected into all animals (4 mg/kg
subcutaneously) just before the operation for pre-emptive analgesia and then every 24
h for 3 days postoperatively. The backs of the animals, in the cervical region of each
animal, were surgically prepared for aseptic surgery. An excision wound of size 4 cm2
was made by cutting out a 2×2 cm piece of skin from the shaved area. The wounds were
of full thickness type extending up to the subcutaneous tissue.