Detoxification process (removal of alcoholic fermentation inhibiting compounds). Detoxification was performed with the use of activated carbon (20 g/100 g of the material) in the temperature of 80 °C in 2 h time (with simultaneous shaking of 150 rpm). Then the lignocellulosic substrate was rinsed with distilled water (7 L of H2O/140 mL of sample) and, after filtering, it was dried in 105 °C temperature for 1 h.