Proteases with high activity and stability at high alkaline
ranges and high temperatures are interesting for bioengineering
and biotechnological applications. Their major application is
in the detergent industry, because the pH of laundry detergents
is generally in the range of 9–12, accounting for about 35% of
total microbial enzyme sales [13,14]. It is reported that in the
year 2005, the global proteolytic enzyme demand for protease
was increased dramatically to 1–1.2 billion dollars [15].
The aim of the present work is to produce protease by Bacillus
licheniformis cells immobilized on chitosan, corn cob and
corn tassle and to optimize enzyme production conditions.