From the experiment on disc diffusion method, all the micro organisms demonstrated maximum zone
of inhibition at 0% dilution of the ethanol extract, i.e., when the betel leaf extract was 100% concentrated it
showed maximum inhibitory effect on the microorganisms. It can also be added that the microorganism Proteus
vulgaris exhibited maximum zone of inhibition at 0% dilution of plant extract at 13mm followed by Klebsiella
at 11.5mm. From the disc diffusion method it could be concluded that Proteus vulgaris and Klebsiella were inhibited to a great extent by the betel leaf extract, followed by Pseudomonas and Staphylococcus aureus (Table
2)