The P. stylifera shrimp shell wastes were collected from the Versova landing centre, Mumbai. Shells are removed and thoroughly washed with running tap water with sample care so as to remove sand adhered to it, the exoskeleton were subjected to shade drying for 2 days and then placed in hot air oven for at 600C for 24 hours. The preparation of chitin from shrimp shell followed by24 with some modification. Diluted HCl solution was used for demineralization. One hundred grams of shrimp shell powder was immersed in 1000 ml of 7% (w/w) HCl at room temperature (25°C) for 24 h. After filtration with mid speed filter paper, the residue was washed with distilled water to neutral.Then the residue was immersed in 1000 ml of 10% (w/w) NaOH at 60°C for 24 h for deproteination. The proteins were removed by filtration. Distilled water was used to wash the residue to neutral. Then the shrimp shell residue was subjected to the above program for two times. 250 ml of 95% and absolute ethanol were sequentially used to remove ethanol-soluble substances from the obtained crude chitin and to dehydrate. An air oven was taken to dry the chitin at 50°C overnight.