Experimental designs
Muscle foetal development. Charolais foetuses were generated
by artificial insemination of Charolais cows with the
semens of two Charolais bulls as reported earlier (Chaze
et al., 2009). They were collected from cows slaughtered at
the Experimental INRA slaughterhouse at pregnancy days 60
(n 54), 110 (n 54), 180 (n 54), 210 (n 54) and 260
(n 54). The Semitendinosus (ST) muscle was excised within
15 min, frozen directly in liquid nitrogen and stored at
2808C until analysis.