EXPERIMENT 2: identification of bacterial contaminants in sugarcane plantlet and rice seedling in TIB system
Bacterial contaminants of TIB plant tissue were isolated directly from visibly contaminated plant culture by spreading on NA containing nystatin. Purified bacteria cultures were characterized by the 16S rRNA gene sequencing. The species of bacterial contaminants in 700 ml and 2L TIB of sugarcane and rice seeding were shown in Fig.4, Fig.5 and Table 1, respectively.