DPPH radical scavenging activity
To measure the DPPH radical scavenging activity of green
tea and lotus extracts, the method as reported by Lee and
Shibamoto [19] was used. For this, 0.1 mM solution of
DPPH was prepared in ethanol. Then 5 μL of the extract
was dissolved in DMSO and mixed with ethanolic DPPH
solution (95 μL). The above mixture was then dispersed
in a 96-well microplates reader (Spectra Max plus 384
Molecular Device, USA) and incubated at 37°C for 30 min
and the absorbance was measured at 515 nm. From this,
the radical scavenging activity was determined by using
the following Equation 2:
DPPH scavenging effect ð%Þ ¼ 1‐ðAc–As=AcÞ 100
ð2Þ
Where Ac = absorbance of control and As = absorbance of sample