2.2. Extraction and quantification of photosynthetic pigments
Chlorophyll and carotenoids were extracted from 10 mg (wet
plant biomass) of floating leaves with 2 mL of dimethyl sulfoxide.
Samples were incubated in darkness for 12 h at 45 ◦C. Chlorophyll
a, b and carotenoids were determined spectrophotometrically at
wavelengths of 665, 649 and 480 nm. Pigment concentrations were
calculated using Wellburn’s formulae [36], and expressed as g g−1
FW.