2.3. Experimental design
Experiments were carried out in 1 L working volume under natural and control condition in three different salinities which
were 20, 30 and 40 ppt. For the maximum density of Nanno-chloropsis oculata, the optimal salinity condition was 30 ppt ac-
cording to Cho et al. (2007). Hence, 20 ppt and 40 ppt salinity were included in this study to see the performance on both species when cultured under variable range of salinity other than the normal 30 ppt seawater salinity.