Enhanced
oxalic
acid
production
was
investigated
in
semi-solid
state
fermentation
of
methanol-resistant
Aspergillus
niger
using
corncob.
Methanol-resistant
A.
niger
was
selected
from
the
sector
of
colony
showing
phenotypically
intact
growth
despite
5%
methanol
in
solid
medium
(PDA)
and
finally
obtained
through
the
adaptation
process
for
22
weeks.
Oxalic
acid
production
of
methanol-resistant
A.
niger
was
dramat-
ically
enlarged
in
liquid
culture
(CZB)
by
9.14
folds
compared
with
that
of
parent
cells.
Semi-solid
state
fermentation
of
corncob
has
been
developed
for
the
application
of
low-cost
and
environment
friendly
biomass
waste
to
organic
acid
production.
Oxalic
acid
production
was
dependent
of
inoculum
size
and
initial
substrate
concentration,
which
was
optimized
at
10 6 spores/g
dry
weight
and
5%
(w/v)
corncob
in
0.1
N
NaOH
solution,
respectively,
which
led
to
the
maximum
productivity
(123.0
g/kg
dry
weight
of
corn-
cob).
Results
demonstrate
that
methanol-resistant
A.
niger
has
great
potentials
for
oxalic
acid
production
from
biomass.