The assay was based on decrease of anodic current of hydrogen peroxide complex, formed in alkaline solution, at the potential of mercury
dissolution. High correlations between honey HPS activity, its total phenolic content (FC-GAE),
antioxidant activity measured by four standard methods (DPPH, TEAC, FRAP and ORAC), and also the
relative antioxidant capacity index, were obtained. Statistical evaluation by ANOVA and F-test further
confirmed the assay validity. The results for individual compounds showed that HPS activity of honey
reflects an integrated action of a wide range of constituents, both phenolics and non-phenolics. The
polarographic assay applied is a fast, reliable and low cost alternative to spectrophotometric antioxidant
assays commonly applied in analysis of honey and can serve as an indicator of honey quality.