In our search for promising lipid molecules for efficient gene transfection, we have described certain cationic lipids where hydrocarbon chains are anchored via aromatic backbone. The nature of the spacer and their hydratability influence the aggregation and transfection properties singnificantly. Gene transfection involves DNA-lipid complexation (lipoplex formation), which is governed by blectrostatic interactions. These interactions are in turn influenced by both the hydration of the lipids during lipoplex formation as well as the spine of hydration at the DNA backbone. Therefore it would be interesting to examine transfection properties of aromatic gemini lipids possessing oxyethylene type spacer.