Reaction mixture
(0.5 ml) containing 0.1 ml (25%, w/v) of rat liver homogenate
in Tris_
HCl buffer (40 mM, pH 7.0), 30 mM KCl
(100 ll), 0.16 mM ferrous iron (100 ll), 0.06 mM ascorbic
acid (100 ll) and the sample solution (100 ll) at various concentrations
were incubated for 1 h at 37 C. The lipid peroxide
formed was measured by thiobarbituric acid reactive
substances (TBARS) values