Plates of potato dextrose agar medium (PDA) were
inoculated with a 7 mm diameter disk of B. oryzae
10 mm from the edge of the plate. A 7 mm diameter
disk of the Trichoderma isolate being tested was
placed 10 mm from the edge of the plate and opposite
the B. oryzae disk. PDA plates inoculated with only a
7 mm diameter disk of either B. oryzae or T.
harzianum 10 mm from the edge of the plates were
used as control treatments. Seven replicates of each
treatment were used. Plates were incubated at
25 ± 18C under alternating luminosity (12 h light/
12 h darkness) for 8 days. The inward linear growth
was measured after 2, 4, 6, and 8 days, and the size of
the inhibition zone and amount of overgrowth of
Trichoderma on B. oryzae was used to measure the
interaction between the two fungi [9]