Surface charge plays an important role in the mechanism of cell attraction. It was also supported by previous study that at neutral pH, microalgae reveal a slightly negative charge because of the presence of proton-active carboxylic, phosphoric, phosphor-diester, hydroxyl, and amine functional groups (Abdul Hamid et al., 2014, Hadjoudja et al., 2010 and Molina Grima et al., 2003). In this study, the zeta potential of surface charge microalgae was reported to be negative within the range from −10 mV to −35 mV while fungus was negative at <−10 mV, which is in agreement with what was reported by other researcher (Murugesan et al., 2014). Thus molecular repulsion of microalgae-fungus complex was likely to be contributed by their charges that force the aggregation of microalgae biomass within the matrix structure of fungus filamentous hyphae.
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