according to the manufacturer’s instructions, with 304 ng of total RNA as a template in a reaction volume of
20 ll. Real-time PCR was accomplished with LightCycler 480 SYBR Green I Master (Roche, Mannheim, Germany), according to manufacturer’s instructions, with 2 ng of cDNA as template in a single reaction. The PCR was carried out and analysed with LightCycler 480 Real-Time PCR System (Roche). For each time point/treatment four biological replicates, consisting of four individuals each, were analysed. All reactions were prepared as triplicates and a no-template control was included for each primer pair.
according to the manufacturer’s instructions, with 304 ng of total RNA as a template in a reaction volume of20 ll. Real-time PCR was accomplished with LightCycler 480 SYBR Green I Master (Roche, Mannheim, Germany), according to manufacturer’s instructions, with 2 ng of cDNA as template in a single reaction. The PCR was carried out and analysed with LightCycler 480 Real-Time PCR System (Roche). For each time point/treatment four biological replicates, consisting of four individuals each, were analysed. All reactions were prepared as triplicates and a no-template control was included for each primer pair.
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