2.5. Oil holding capacity
The oil holding capacity (OHC) of unripe banana flour was
determined according to the methods of Larrauri, Ruperez,
Borroto, and Saura-Calixto (1996). Approximately 1 g of banana
flour was weighed into 15 mL centrifuge tubes and thoroughly
mixed with 10 mL of cooking oil. Samples were allowed to stand
for 30 min and centrifuged at 3000 rpm for 20 min. After centrifuging,
the supernatant was poured into a 10 mL graduated cylinder
and the volume recorded. Final OHC values were calculated thus: