The PCR program was as follows:94 ◦C for 1 min, and then 30 cycles of 94 ◦C for 40 s, 55–62 ◦C
for 40 s and 72 ◦C for 40 s), followed by a final 7-min extension at 72 ◦C in PCR Thermal Cycler Dice (Takara Bio, Shiga, Japan). Amplified products were analyzed by electrophoresis. Optimized
detection methods were as follows