Many culture media have been developed for the selective
enumeration of bifidobacteria, being basically composed of the medium
de Man, Rogosa & Sharpe (MRS) supplemented with different
antimicrobial compounds or their combinations (Dave and Shah,
1996; Leuschner et al., 2003; Lima et al., 2009). In 2010, the International
Organization for Standardization published ISO 29981, a protocol
for the enumeration of bifidobacteria in fermentedmilk which
employs a culture medium containing transgalactosilated oligosaccharide
(TOS) and lithium mupirocin as an inhibitor (ISO, 2010).