The reproducibility of the automated rep-PCR was examined by comparing DNA profiles from the recovered M fortuitum strains. Two rounds of DNA extraction and 5 replicates of rep-PCR were performed, and the profiles obtained from these analyses were similar or undistinguishable. In addition, water samples were collected, and M fortuitum was recovered in water samples from 3 sites outside the department of gynecology. This supported the presence of M fortuitum in the water supply for the whole hospital.