The buoyant density of CTFV in CsCl is 1.36–1.38 g/cm3. The virus is stable between pH 7.0 and 8.0 but loses infectivity at pH 3.0. CTFV can be stored at 4°C for 2–3 months in 50% fetal calf serum, 0.2 mmol/L Tris-HC1, pH 7.8, or for years at –80°C. Upon heating to 55°C, CTFV loses its infectivity.