2.4. Taxonomic characterization of antagonistic isolates TB09 and
TB072
The isolated bacteria with presumptive volatile antifungal
activity were identified based on morphological phenotype and
16S rDNA sequence analysis. The sequences for the primers are
27F: (50-AGAGTTTGATCCTGGTCAGAACGCT-30) and 1492R: (50-
TACGGCTACCT TGTTACGACTTCACCCC-30). Bacterial DNA was isolated
from each strain using the established CTAB method as described
in Jin et al. (2011). The resulting PCR products were
purified and used directly for sequencing (Invitrogen, Shanghai,
China). Homology search comparisons were carried out using the
database available at NCBI BLAST (http://www.ncbi.nlm.nih.gov/).