. This research was conducted to study the effects of two explant types (leaf disc and petiole) and phytohormones including
5 levels of Benzyl Adenine (BA) (0, 0.2, 0.5, 1, 2 mgL-1) and 2 levels of Indole Butyric Acid (IBA) (0 and 0.5 mgL-1) onin vitro
micropropagation of African violet (Saintpaulia ionantha). Based on primary data, experiments were performed with Murashige and
Skoog`s basal media in factorial design was arranged in complete randomized blocks with four replications. Results showed that the
number and length of adventitious shoots were higher in leaf discs compared with leaf petioles. It was also revealed that elimination
of IBA as an auxin source decreased the regeneration efficiency and prolonged the time required for shoot regeneration. Generally,
the highest numbers of adventitious shoots (80 shoots per explant) were observed on a medium containing 0.5 mgL-1 BA along with
0.5 mgL-1 IBA. Among the different hormonal combinations, the medium supplied with 0.2 mgL-1 Gibberellic Acid (GA3) caused the
maximum shoot elongation with an average length of 19.9 mm. The half strength MS medium containing 1 mgL-1 IBA lead to
formation of normal plants with longer roots in micropropagated shoots. The rooted shoots were then acclimatized under high
humidity (80-90% relative humidity) in a mixture of peat moss and perlite (3:1) for three weeks with a survival rate of about 95 %.