The cytomorphologic details of tissues were evaluated by way of variables described Table/Fig-2) to assess the effect of acid treatment in the three techniques used. Modified methods using heat and mechanical agitation showed higher proportion of artefacts in bone in terms of folds (p 0.5) marrow cell nuclear details (p 0.201). However, the method employing stirring demonstrated less osteocyte retraction (p 0.045); TTable/Fig-4,5] On assessment of teeth specimens decalcified by the three methods, it was seen that nuclear-cytoplasmic contrast of pulp cells significantly deteriorated on both heating and stirring wherein most specimens demonstrated only average nuclear-cytoplasmic contrast. All teeth specimens decalcified by the conventional technique exhibited good nuclear-cytoplasmic contrast (p 0.021 [Table/Fig-6). However, no significant difference was seen in the amount of pulp shrinkage between the three methods. A trend for increased pulp shrinkage was noted with the stirring technique was also noted that the odontoblast layer was distinct in 20% of the specimens subjected to decalcification by heat in contrast 80% and 60% in the conventional and stirring methods respectively Table/Fig 4] soft tissue sections, increased eosinophilia was seen in the conventional and stirring methods, with intense eosinophilia in all cases in the latter technique (p 0.162). There was no significant difference in nuclear contrast and chromatin visualisation between the three methods [Table/Fig-4).