*
Means ± S.D. from triplicate determinations. **Different superscripts in the same row indicate the significant differences (p < 0.05). Number of observations = ***10. Bromelain extract (BE) was obtained from the top phase of aqueous two phase system comprising of 18% PEG-6000 and 17% MgSO4. The muscle samples were
treated with difference concentrations of BE and then subjected to incubate at room temperature for 60 min. before properties measurement.