Various parameters such as KOH loading, catalysts to oil ratio and reaction time greatly influence the grandest edification reaction.
Among the different catalysts used, ZSM5 loaded with 35 at. % of KOH showed a very high catalytic activity (>93% of FAME yield) at the optimum determined reaction conditions: methanol to oil molar ratio of 12:1, catalysts amount of 18 wt. %., and a reaction time of 6 h.