In conclusion, a reliable reference QRT-PCR assay was first confirmed
to be specific to the specie in question, and second, a uniform
positive signal among different matrix samples containing
the same species was given. With respecting the establishment criteria
of a reference gene, the developed real-time PCR method is
both reliable and suitable for the detection and quantification of
reference genes in food samples. To our knowledge, this is the first
report on a QRT-PCR assay aimed at the specific detection of the S.
salar in food to be used for GM Aquadvantage quantification