Introduction
Micropropagation is a massal culture system
developed
under
conditions
of
asepsis, high
humidity and controlled lightness and temperature.
Acclimatization is a critical stage in micropropagation.
During this period the higher
percentages of plant losses occur due to several
reasons (Kozai, 1991; van Huylendroeck et al.,
1998). This is the reason why is necessary to obtain
quality
plantlets
under
in
vitro
conditions
to
ensure
a
high
survival
percentage
and
an
appropriate
growth under greenhouse conditions. In
order to survive ex vitro a plantlet must achieve
a growth stage with an appropriate sprout number,
foliage area and radicular system, considering
roots
number
and length.