2.3.2. Fed batch mode
The fed-batch SSF experiments were started with an initial 19%
DM. Another 6% DM was added in 3, 6, and 12 portions during the
first 24 h, giving a final DM concentration of 25%. The fermentation
conditions, enzyme loadings, and nutrient concentrations were the
same as in the batch experiments. Cellulase loading was 22.8 FPU/g
glucan.