2.4 Determining the appropriate basal media on young leaf culture
The best results of the first two studies were used as the basis for studies of four media. Basal leaf segments were laid horizontally on four basal media; VW, NDM, Murashige and Skoog (MS) and Ichihashi and Yamashita (IY) (Table 1) supplemented with 0.1 mg/l NAA and 1 mg/l BA. Five replicates with eight explants were used for each treatment. Treatment means were compared by The Least Significant Difference.