2.4. Preparation and assay of enzymes involved in starch synthesis
The panicles stored at 70 C were de-hulled manually, and the
fresh weight of 20 grains was recorded. The enzymes were
extracted and analysed according to the methods described by
Chen, Jiang, Wu, and Shi (2001). Four key enzymes in the starch
synthesis process, namely ADP-glucose pyrophosphorylase (AGP),
starch synthase (SS), starch branching enzyme (BE), and debranching
enzyme (DBE), were analysed. All of the procedures were
performed in triplicate and on ice.