The effects of enzyme modulators
{ascorbic acid,ethylenediamine-tetraacetic acid (EDTA), b-mercaptoethanol, DTT and urea at a final concentration of 5 mM}
on the PSB phytase activity was measured in the reaction mixture as described ahead.
Enzyme solution (100 ll) was pre-incubated with 900 ll acetate buffer with respective modulator for 30 min at 37 _C,
followed by the standard enzyme assay as described earlier.
The activity assayed in the absence of the modulators was taken as the control.