The focus will be on sample preparation for the commercial platforms already on the market, in particular for
the 454 Genome Sequencer FLX (henceforth termed the ‘FLX’), the Illumina Genome Analyzer IIx (‘GA’, used loosely to include the recent HiSeq 2000) and the Applied Biosystems SOLiD 3 Plus (‘SOLiD’), although many general principles will apply also to emerging and future systems. Enrichment methods, used to selectively enrich desired target regions in a genome, have been reviewed elsewhere [3,6] and will not be covered here.
The focus will be on sample preparation for the commercial platforms already on the market, in particular forthe 454 Genome Sequencer FLX (henceforth termed the ‘FLX’), the Illumina Genome Analyzer IIx (‘GA’, used loosely to include the recent HiSeq 2000) and the Applied Biosystems SOLiD 3 Plus (‘SOLiD’), although many general principles will apply also to emerging and future systems. Enrichment methods, used to selectively enrich desired target regions in a genome, have been reviewed elsewhere [3,6] and will not be covered here.
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