High Performance Liquid Chromatography
In accordance with the international standards of the
Organic Crop Improvement Association (OCIA), the
International Federation of Organic Agriculture Movements
(IFOAM) and the National Organic Program of
the U.S. Department of Agriculture, the use of environmentally
friendly extraction procedures, such as maceration,
is needed to obtain such natural ingredients.
We used an objective HPLC assay coupled to spectrophotometric
methods for exhaustive characterization of
polyphenolics, including compounds with antioxidant
potential such as flavanols, phenolic acids and carotenoids,
from an extract of C. officinalis flowers. The most
abundant phenolics were quantified using HPLC by
comparison with external standards of known phenolics.
High Performance Liquid ChromatographyIn accordance with the international standards of theOrganic Crop Improvement Association (OCIA), theInternational Federation of Organic Agriculture Movements(IFOAM) and the National Organic Program ofthe U.S. Department of Agriculture, the use of environmentallyfriendly extraction procedures, such as maceration,is needed to obtain such natural ingredients.We used an objective HPLC assay coupled to spectrophotometricmethods for exhaustive characterization ofpolyphenolics, including compounds with antioxidantpotential such as flavanols, phenolic acids and carotenoids,from an extract of C. officinalis flowers. The mostabundant phenolics were quantified using HPLC bycomparison with external standards of known phenolics.
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