2.9. Desulfation
Sulfate content was determined as described previously (Ghosh et al., 2004). Polysaccharides (OSP and OF4; 150 mg) were desulfated according to Nagasawa, Inoue, and Kamara (1977) and the desulfated polysaccharides (OSP-D and OF4-D, respectively) were isolated by freeze-drying (62 and 71 mg).