Nile Red (Sigma-Aldrich, USA) diluted in
dimethylsulfoxide (DMSO) (0.25 mg/mL) was filtered and
added to the sterile MM medium. The tested strain was
cultivated into agar plates at 30 ºC for 72 hours. The agar
plates were exposed to UV light (312 nm) after cultivation to
detect PHA granules inside the cells. The cells were
subsequently observed under fluorescent microscopy
(Labophot Microscope, Nikon Instruments, New York,
USA).