A previously described method was applied to determine the antagonistic activity of isolates against clinically important human pathogens [33]. Table 2 lists the indicator pathogens. A total of 2 ml of overnight cultured bacteria in the MRS broth was filtered using Nalgene syringe filter units (0.2 mm). A total of 50 mL of filtrates was then poured into 7 mm diameter wells created in the Muel- lereHinton agar plates, which contained the mentioned indicator pathogens. The clear zone diameter for each isolated strain was measured and analyzed after 24 h incubation at 37 C. The areola diameter was an indicator of anti-pathogenic activity. Antagonistic activity was divided into three groups: strong (diameter !20 mm), moderate (20 mm < diameter > 10 mm), and weak (diameter 10 mm)