This experiment extract of phenolic compound was determined by using Folin- ciocalteu assay, a widely method for estimation of plant phenols by color yield, spectrum 760 nm, time- temperature effect and interference (Singleton and Rossi,1965) and record graph by stand calibration curve of gallic acid. This graph absorbance760 nm. Concentration of gallic acid is increase according. Phenolic ions generate the reagent change from yellow to dark blue color.
From the standard calibration curve of gallic acid solution you can see the color of solution change from yellow change to dark blue green. Therefore when you add gallic acid increase, the color of solution will change to darker blue color.
From the Extract concentration you can see the color of solution change from colorless to dark blue .Therefore when extract concentration decrease, the color of solution will darker blue. But we found that values absorbance of the solution extract concentration 10mg/mL, 0.1mg/mL, 0.01mg/mL, 0.001mg/mL is error except extract concentration 1mg/ml.